circular m13mp18 ssdna (New England Biolabs)

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New England Biolabs circular m13mp18 ssdna
Circular M13mp18 Ssdna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 873 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/circular+m13mp18+ssdna/M13mp18+Single-stranded+DNA/pmc13081101-136-10-14
Average 96 stars, based on 873 article reviews

circular m13mp18 ssdna - by Bioz Stars, 2026-10

96/100 stars

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Construct:

Article Title: Light-Guided Molecular Patterning for High-Throughput Single-Molecule Mechanical Characterization.
Article Snippet: .. The DNA nanoswitch construct for force spectroscopy is synthesized with circular M13mp18 ssDNA (N4040S, New England Biolabs). ..

Article Title: Light‐Guided Molecular Patterning for High‐Throughput Single‐Molecule Mechanical Characterization
Article Snippet: .. The DNA nanoswitch construct for force spectroscopy is synthesized with circular M13mp18 ssDNA (N4040S, New England Biolabs). ..

Article Title: Light-guided molecular patterning for programmable multiplexed single-molecule manipulation
Article Snippet: .. The DNA nanoswitch construct for force spectroscopy is synthesized with circular M13mp18 ssDNA (N4040S, New England Biolabs). ..

Force Spectroscopy:

Synthesized:

Blocking Assay:

Article Title: DNA damaged-induced phosphorylation of a viral replicative DNA helicase results in inhibition of DNA replication through attenuation of helicase function
Article Snippet: End-labelled, annealed substrate was purified with illustra ProbeQuant G-50 Micro Column (GE Healthcare). γ 32 P ATP-labelled 17mer helicase substrate was created by end labelling 0.05 nanomoles of a short oligo (5’-GTAAAACGACGGCCAGT-3’) with γP 32 ATP using T4 Polynucleotide Kinase according to the manufacturer’s protocol (Fermentas). .. End-labelled oligo was then mixed with equimolar amount of unlabeled, circular M13mp18 ssDNA (NEB), placed at 95°C for 5 minutes, and the heat block was removed to room temperature to allow substrate to anneal slowly overnight. .. End-labelled, annealed substrate was purified with illustra ProbeQuant G-50 Micro Column (GE Healthcare).

Article Title: DNA damage-induced phosphorylation of a replicative DNA helicase results in inhibition of DNA replication through attenuation of helicase function
Article Snippet: End-labelled, annealed substrate was purified with illustra ProbeQuant G-50 Micro Column (GE Healthcare). γ 32 P ATP-labelled 17mer helicase substrate was created by end labelling 0.05 nanomoles of a short oligo (5′-GTAAAACGACGGCCAGT-3′) with γP 32 ATP using T4 Polynucleotide Kinase according to the manufacturer's protocol (Fermentas). .. End-labelled oligo was then mixed with equimolar amount of unlabeled, circular M13mp18 ssDNA (NEB), placed at 95°C for 5 min, and the heat block was removed to room temperature to allow substrate to anneal slowly overnight. .. End-labelled, annealed substrate was purified with illustra ProbeQuant G-50 Micro Column (GE Healthcare).

Article Title: DNA damage-induced phosphorylation of a replicative DNA helicase results in inhibition of DNA replication through attenuation of helicase function.
Article Snippet: End-labelled, annealed substrate was purified with illustra ProbeQuant G-50 Micro Column (GE Healthcare). γ32 P ATP-labelled 17mer helicase substrate was created by end labelling 0.05 nanomoles of a short oligo (5 ′ -GTAAAA CGA CGGCCA GT-3 ′ ) with γP 32 ATP using T4 Polynucleotide Kinase according to the manufacturer’s protocol (Fermentas). .. End-labelled oligo was then mixed with equimolar amount of unlabeled, circular M13mp18 ssDNA (NEB), placed at 95 ◦C for 5 min, and the heat block was removed to room temperature to allow substrate to Figure 2. ..

Incubation:

Article Title: Lifetime of glass nanopores in a PDMS chip for single-molecule sensing
Article Snippet: .. Circular M13mp18 ssDNA was linearized by hybridizing a 39 nucleotides (nt) ssDNA oligo (5′-TCTAGAGGATCCCCGGGTACCGAGCTCGAATTCGTAATC-3′) then it was cut using the following reaction mixture: i) 40 μL M13mp18 ssDNA (250ng/μL), 8 μL 10x Cutsmart buffer (New England Biolabs), 2 μL of the 39 nt the oligo (100μM) and 28 μL deionized water; ii) then the mixture was heated to 65°C and linearly cooled to 25°C in a thermocycler over 40 minutes; iii) 1 μL of BamHI-HF and 1 μL EcoRI-HF were added (100000 units/ml, New England Biolabs); and iv) then incubated at 37°C for 1 hour. .. Linear M13mp18 was purified with a Machery-Nagel NucleoSpin Gel and PCR Clean-up kit before measuring its concentration with NanoDrop.

Generated:

Article Title: N- and C-terminal tandem tag system for purification
Article Snippet: .. Single-Molecule Flow-Stretching Bead Assay The primed single-stranded DNA (ssDNA) substrate was generated by annealing circular M13mp18 ssDNA (New England BioLabs) to 100-fold excess of the 5′-Digoxigenin-CTAGAGGATCCCCGGGTACCGAGCTCGAATTCGTAATCA-Biotin-TGGTCATAGCTGTTTCCTGTGTG-3′ (SEQ ID NO:25) primer (Integrated DNA Technologies), which contains two orthogonal attachment modifications and which upon hybridization to M13mp18 ssDNA generates an EcoR1 restriction site. .. The annealed DNA was linearized with EcoRI (New England BioLabs), and the reaction was stopped by EcoRI inactivation at 65° C. for 20 min.

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circular m13mp18 ssdna (New England Biolabs)

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